The Protein Expression core laboratory is a service laboratory whose primary goal is to assist investigators at Indiana University in protein structure-function studies. The experienced personnel of the core provide assistance in the selection of an optimal recombinant expression system (prokaryotic and eukaryotic), subcloning genes of interest into expression vectors, and in optimizing protein expression and purification. The core also assists in the generation of site-directed mutants of the proteins of interest and in scaling up of recombinant protein production. The core is equipped to express proteins in E.coli and insect cells. Among the equipment in the core are a New Brunswick Bioflow 3000 Fermentor that has the capacity to run 10 Liter fermentation, a Thermo Spectronic French Pressure Cell Press, Pharmacia FPLC and Bio-Rad Biologic LP protein purification systems.